Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. No additional manuals are available. For readers without a cuvette port, these options are not shown in the software. 6 for further experiment, so I'm confused which i would choose for OD value determination. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 1. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. limit of detection (LLD) for ATP was estimated for each microplate reader using the . 313 reference gain check fail. . Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. We would like to show you a description here but the site won’t allow us. Examples: “Press carefully to engage,” “ Do not. Examples: “Press carefully to engage,” “ Do not press down. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. With optimized reagents, option to operate the. Offer Ends In: 2 Days 10: 40: 19. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. . B: Selection of Excitation Wavelength. SpectraMax Microplate Readers. The dual monochromator optics allow. Open the top cover of the instrument. Products : General, Microplate Readers. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). FI assay. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. com. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. For readers without a cuvette port, simply check the box next to ‘PathCheck’. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. Fluorescence Microplate Reader Comparison Chart. Use the provided 3. Download the SpectraMax Mini Reader Plug-in and associated guides. Table of Contents. 314 low lamp level warning. See SpectraMax® Microplate Reader User Manual Download Page. Create custom fluorescence assays without having to purchase expensive filter sets. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0 out of 5 $ 2,999-20%. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. These lab workhorses are used in drug discovery, research, bioassay. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. begin reading microplates with seven mouse clicks. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Measure more accurately. Step 1: Turn on your microplate reader. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Request a Quote!Introduction. microplate reader. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Table 1. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. In addition, the iD5 reader measures TRF and FP. flat meniscus), but is approx 15 mL. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Highly customizable low volume. Transcreener ® ADP. 100 readings per well gives optimal Z’ values for this assay. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 470 nm). Page 28 Chapter 5: Read Modes and Read Types 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. For a higher . Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Ease of use. Selection of Water Constant in PathCheck settings. A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Microplate Readers Biotek Epoch Microplate Reader. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. It features a modular design that allows users to add or change detection cartridges according to their needs. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Sale. Molecular Interactions. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Microplate reader settings. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. Endpoint, kinetic, spectrum. The dual monochromator optics allow the. 315 can't find zero order. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Temperature control. We would like to show you a description here but the site won’t allow us. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Example: [Drawer]. Unlock your i3/i3x instrument after shipment. Supplier: Molecular Devices. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Guides: SpectraMax Mini Installation Guide. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. This unlocks the transport slide. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Please refer to the Obsolete Microplate Reader Models for more information. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Brochure. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. Italic and boldface type are used for emphasis. , April 14, 2022 – Molecular Devices, LLC. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. The plate. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. . Microplate Spectrophotometers. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. zip. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Italic and boldface type are used for emphasis. Bookings are free, but you will need to use iLab to book. Molecular Devices. Materials. flexibility of monochromator-based systems. For SpectraMax ABS Plus reader, r 2 = 0. Nuclear Receptor. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Introduction. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Read More. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. ATP standard curves read on SpectraMax microplate readers. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 997 (n = 3). Published Dec 16, 2020 | Updated Dec 16, 2020. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. 100 fmol 1536-well (8 µL) 0. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Fill a clean plate with water. . Introduction. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. Using the. The dual monochromator optics allow the widest range of. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . It also integrates with the. The SpectraMax M2 and M2e are versatile and reliable. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. no. Additionally, user-installable options allow the SpectraMax i3 System to. Detailed specifications of each model reader are reported in Section 7, “Specifications”. All . Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. The CellTiter-Glo assay from Promega uses luciferase. . An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Offer Ends In: 18 Days 19: 38: 21. SpectraMax M5 reader settings are shown. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. . In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Avoid losing high signal. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. western blot membrane detection in a multimode microplate reader platform. Three low volume 24-well bottom slides. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. Selection of Water Constant in PathCheck settings. 1. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Microplate Readers. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. This pathlength correction is accomplished only with the use of. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Results are shown in Table 1. Examples: “Press carefully to engage,” “ Do not. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. These lab workhorses are used in drug discovery. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. 6% for the on-board optics (monochromators). It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Type: Clear: Add to Cart. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. SpectraMax 250 User. 303 unable to cal dark current. Add to Cart. Assays vary depending on. Molecular Devices. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Endpoint, kinetic, spectrum. microplate reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. View the Molecular Devices website. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. com 4. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Data exemplifying linear ranges from the three different kits are presented in Figure 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. 7°C (gradient heating design). Microplate automation and detection instruments for walk away operation and high quality results. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. . Blank subtraction and standard curve fitting were done automatically by the software. In . Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. 1. Omnia® Compatible Microplate Reader Documentation Version No. An assay plate containing a 15-point standard curve was read on the SpectraMax. SAN JOSE, CA — Molecular Devices, LLC. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Compact designs. We would like to show you a description here but the site won’t allow us. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. flexibility of monochromator-based systems. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). 1 software Download page. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Other technologies such as imaging. The calculated LOQ values were 0. All stand-alone instrument functions can be controlled using the software. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 012 fmol 0. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. 2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Example: [Drawer]. 40266 Email: drugdiscoverytech@lifetech. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. More Product Information. With optimized reagents, option to operate the. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 20 μL of Component A was added to each well of the microplate. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 2. Thank you!. Cell Analysis. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. Assays can be better optimized by scanning across. Turn the reader off by pulling the power cord. Microplate readers’ standard detection modes. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Example: [Drawer]. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Contents of wells were pipetted up and down several times to mix. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Align the two slots on the bottle holder with the two pegs on the rail. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. both cuvette-based and microplate reader applications on the same instrument. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Or scan up to 384 wells at any time to determine the best assay conditions. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. Time-. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. Most papers they cultured bacteria to OD600>0. Or, with the reader specifications noted from the Instrument Description section of your reader's. Figure 1. Download PDF. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. On the back, in the right hand top corner,. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Plate chamber design of the SpectraMax category 1 microplate readers. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it.